Csf manual cell count procedure

CEREBROSPINAL FLUID (CSF) CELL COUNT AND DIFFERENTIAL. Author(s), Name Title Penny S. Stevens MBS, MT (ASCP), CLS (NCA) Document Number Effective Date Sr. International QA/QC Coordinator ProE Review by Amy Rada Review date pSMILE Comments: This document is provided as an example www.doorway.ru: Penny S. Stevens. Reference range for cell counts CSF obtained by lumbar puncture: WBC cells Depending on the medical condition, CSF may also contain non-haematopoietic cells (e.g. tumour cells, astrocytes, oligo-dendrocytes, etc.). A deliverable of an automated cell count. By counting cells in the 5 shaded squares in both counting chambers (for a total of 10 squares), the standard formula is simplified so that cells/µL = number of cells counted x dilution factor. Differential Cell Count The types of cells present in a CSF specimen provide valuable clues to the etiology of disease, and forFile Size: 29KB.

In general, CSF should be collected into 4 separate allotments with the tubes labeled in order of collection. Tube 1 - saved for possible cell counts to help distinguish a traumatic tap from a subarachnoid hemorrhage. This is routinely required by the Emergency Department; Tube 2 - used for chemistry and immunological studies. Compare the cytospin manual differential and total cell count results. They should correlate as follows: If they do not correlate: Verify the cytospin was prepared using the same dilution as the hemocytometer cell count. Verify calculations. Repeat the hemocytometer cell count. Prepare a new cytospin specimen and repeat the manual differential cell count. Container Type CSF sterile plastic tube. Specimen Type CSF Preferred Volume 1 mL Collection Procedure If three sterile tubes are collected, tube #3 should be sent for total cell count and differential. Specimen Processing Transport ASAP. Fluids delayed more than 2 hours should be refrigerated to a maximum of 72 hours.

further cell count method comparison analysis as Routine testing of CSF included manual cell counting and biochemistry analyses in centrifuged sample. ١٥‏/٠٣‏/٢٠٢١ However, these analyzers have some limitations. – CSF analysis requires previous specimen dilution and preparation, which increases laboratory. ١٨‏/٠٢‏/٢٠٢١ Manual enumeration of WBC's and RBC's and differential cell count (percentage of each cell identified) from a cytocentrifuged, stained slide.