Hitrap q ff column manual

 · HiTrap Q FF, (5 x 1 ml) Column name. HiTrap rProtein A FF, (5 x 1 ml) Manual sample injection is selected in the Sample Injection page in the Method Wizard. HiTrap affinity columns Instruction manual 1 * Special pack delivered on specific customer order. Description Medium properties HisTrap HP 1 ml and 5 ml columns are packed with 1 ml and 5 ml (Q B, 10% determination) or (His) 6-tagged protein bound from E. coli extract. For sealing bottom of HiTrap column 2, 5 or 7. AI 5 Table 2. Characteristics of SP Sepharose XL and Q Sepharose XL ion ex-changers. 1 Determination of dynamic binding capacity: Q Sepharose XL and SP Sepharose XL: Samples were applied at cm/h until 10% breakthrough. Column: × 10 cm.

HiTrap ® Q FF is prepacked with Q Sepharose ® Fast Flow, and is a strong anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions. Q, SP, DEAE, and CM Sepharose ® Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. The columns are delivered with a stopper at the inlet and a snap-off end at the outlet. Table 1 lists the characteristics of HiTrap columns. Fig 1. HiTrap, 1 mL column. Fig 2. HiTrap, 5 mL column. Note: HiTrap columns cannot be opened or refilled. Note: Make sure that the connector is tight to prevent leakage. Table 1. Characteristics of HiTrap. Characteristics of HiTrap DEAE FF, CM FF, Q FF, SP FF and ANX FF columns. 1 At room temperature in water. Note: The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the resin, sample/liquid viscosity and the column tubing used. Supplied Connector kit with HiTrap column Resin Properties Q.

instructions HiTrap HiTrap HiTrap HiTrap HiTrap HiTrap DEAE FF, 1 ml and 5 ml HiTrap ion exchange columns HiTrap™ SP FF, HiTrap CM FF, HiTrap Q FF. Using HiTrap Q HP and HiTrap SP HP prepacked columns is simple. Easy-to-follow instructions are included for fast start- up and method optimization. Whether you. Normally we use DEAE-Sepharose FF and sometimes Q-Sepharose FF columns. With AKTA system use HiTrap DEAE FF or HiTrapQ HP cartridges, just screw 2 to 4 of.